Mycoplasma gallisepticum k kit (elisa)

Hanyar Elisa:

1) Takeauki pre - Micrated Micrroas (Iya Unseal don amfani da lokaci da yawa kamar yadda kowane adadi na samfurin), da yawa saita 1 Dillila don mummunan iko, 2 rijiyar don ingantaccen iko daban. Faɗa 100μL mara kyau / iko mai kyau ga rijiyoyinsa. Shake taushi, kar a ambaci, murfin da kuma shiryawa da minti 37 ℃ don mintina 30.

2) Zuba ruwa daga rijiyoyin, ƙara 250 μl diluted wanke buffer mai wanki ga kowane rijiyar, zuba. Maimaita 4 - sau 6, a ƙarshe pat don bushe akan takarda mai narkewa.

3) Favelara Fasaha 100μl enzyme conjugate ga kowane rijiya, shake da taushi, murfin da kuma murfin da karfe 37 ℃ for 30 min.

4) Maimaita matakin 2 (wanka). Ka tuna Pat ta bushe a kan takarda mai narkewa a ƙarshe.

5) Mara 100μl substrate zuwa kowane rijiya, Mix da kyau, amsa na 10 min atdark A37 ℃ cikin duhu.

6) Addara 50μl na dakatar da bayani a kowane rijiyar, kuma auna sakamakon a cikin min 10 min.

Bayanin samfurin:

A Mycopasma Gallisepticum (MG) Ka'idodi Elisa Kit ya dogara da ingantaccen rigakafi na enzymaty (kai tsaye Elisa). Lokacin da samfurin ɗan ƙaramin abu ya ƙunshi takamaiman abubuwan rigakafi game da kwayar cuta, za su ɗaure ga antigen a kan faranti. Wanke maganin mara tushe da sauran abubuwan haɗin. Sa'an nan kuma ƙara takamaiman enzyme conjugate. Bayan shiryawa da wankewa, ƙara tmp substrate. Halittar launi mai launi zai bayyana, an auna shi ta hanyar Spectrophotometer (450 NM).

Roƙo:

This kit is used to detect Mycoplasma Gallisepticum(MG) antibody in chicken serum, to assess antibody condition by Mycoplasma Gallisepticum(MG) vaccine in chicken farm and assist diagnosis of serological infected chicken.

Adana: Adana da 2 - 8 ℃ cikin duhu.

Ka'idojin zartarwa:Ka'idojin kasa da kasa.