Caf - BMA │ Caffeine BSACUGHANT

Nā Hōʻoia Pūnaewele:

ʻO ka caffeine kahi'ōnaehana paʻakikī o ka'ōnaehana i loaʻa i ka kofe, kīʻaha, a me nā mea inuʻona. Hoʻonui ia i ka makaʻala a hōʻemi i ka momona akā hiki ke hōʻeha i nā hopena e like me ka wā e hopohopo ai, a hoʻonuiʻia i ka nui o ka naʻau i ka nui.

Molecularʻano:

Hapten: Protein = 20 - 30: 1

Nā noi noi:

Ho'ōlaʻo hope i IMMANOOAY, ELisa

Ua'ōleloʻia:

Noi no ka hopuʻana,ʻelua me MD00701 no kaʻike.

'Ōnaehana Bufer:

0 0 0Mha PB, pH7.4

Respsons:

Eʻoluʻolu eʻike i ka palapala hōʻoia o ka loiloi (COA) no ka hoʻounaʻiaʻana me nā huahana.

Ka Hoʻoulu:

ʻO ka Antibody ma kahi o keʻano wai i laweʻia ma ka puka brozen me ka hau polū.

Huanui:

No ka hoʻolimalima lōʻihi, ua paʻa ka huahana no nā makahikiʻelua e mālamaʻia ma - 20 ℃ a haʻahaʻa paha.

Eʻoluʻolu e hoʻohana i ka huahana (ʻano wai) i loko o 2 mau pule inā mālamaʻia ma 2 - 0 ℃.

Eʻoluʻolu e pale i nā manuahi.

Eʻoluʻolu e kelepona iā mākou no kekahi hopohopo.