Havu - Gp36

Nā Hōʻoia Pūnaewele:

HIV, a iʻole he kanaka hanaʻo Rathudeficicy ʻO kēia ka hopena o ka holomua o ka hopena o ka'ōnaehana o ka immune i nā mea hanaʻole, ke hana nei i nā mea āpau i hiki i nā kūpono kūpono a me kekahi mau mea hopu. Ua hoʻounaʻia ke ahonui ma o ka hoʻopiliʻana i ke koko i holoiʻia,ʻo nā manu, ka waiū i ka wā hana maʻamau o ka hoʻounaʻana.

Nā noi noi:

Ho'ōlaʻo hope i IMMANOOAY, ELisa

Ua'ōleloʻia:

No ka noiʻana i loko o ka pālua - Antigen Sandwich no kaʻike,ʻelua me Ai00515 no ka hopuʻana.

'Ōnaehana Bufer:

50mm Tris - HCL, 0.15m Nacl, pH 8.0

Respsons:

Eʻoluʻolu eʻike i ka palapala hōʻoia o ka loiloi (COA) no ka hoʻounaʻiaʻana me nā huahana.

Ka Hoʻoulu:

Ua laweʻia nā proteins i nā mea kūʻai aku ma keʻano o ka wai i laweʻia ma ka puka brozen me ka hau polū.

Huanui:

No ka hoʻolimalima lōʻihi, ua paʻa ka huahana no nā makahikiʻelua e mālamaʻia ma - 20 ℃ a haʻahaʻa paha.

Eʻoluʻolu e hoʻohana i ka huahana (pepa wai wai a iʻole lyophilized paha ma hope o ka heluʻana ma hope o 2 mau pule inā mālamaʻia ma 2 - ℃.

Eʻoluʻolu e pale i nā manuahi.

Eʻoluʻolu e kelepona iā mākou no kekahi hopohopo.

MOOKAI KAIKOA:

ʻO ka wahineʻo Dersusfielsey ʻO ka membrane waho o ka virus he envelope kahi envelope e loaʻa ana i nā protein proteins viral gp120 a me GP41. ʻO GP41 kahi protein transmembrane. Aiaʻo GP120 ma luna o ka maka a me nā kaulahao i GP41 e N non -ʻO ka hui pūʻana. I loko o ka mea i hanaʻia e ka spiherical i hoʻokumuʻia e ka protein p17 a me ka semi - copical copsid i hoʻokumuʻia e ka protein p24.