Kanaka ace2 - kāna mea i koho muaʻiaʻo Raloutensin.ʻO ka hoʻololiʻana i ka enzyme 2 me kāna pahuhopu

Nā Hōʻoia Pūnaewele:

ʻO Angiotelsin - E hoʻohuli i ka enzyme 2 (Ace2) he mea hoʻoikaika nui i pili i ka Renain - RAS) a me nā ROS) a me nā RES) a me nā ROS) a loaʻa nāʻano he nui. Hōʻikeʻiaʻo Ace2 i nāʻano kanaka likeʻole a me nā papa kelepona, me nā pae kiʻekiʻe loa i loaʻa ma ka'ōpū Aia ma kahi o n - Terminal Pepdidase e pili ana i nā hiʻohiʻona o M2 Metallorotses a me kahi C -

Nā noi noi:

Ho'ōlaʻo hope i IMMANOOAY, ELisa

'Ōnaehana Bufer:

50mm Tris - HCL, 0.15m Nacl, pH 8.0

Respsons:

Eʻoluʻolu eʻike i ka palapala hōʻoia o ka loiloi (COA) no ka hoʻounaʻiaʻana me nā huahana.

Ka Hoʻoulu:

Ua laweʻia nā proteins i nā mea kūʻai aku ma keʻano o ka wai i laweʻia ma ka puka brozen me ka hau polū.

Huanui:

No ka hoʻolimalima lōʻihi, ua paʻa ka huahana no nā makahikiʻelua e mālamaʻia ma - 20 ℃ a haʻahaʻa paha.

Eʻoluʻolu e hoʻohana i ka huahana (pepa wai wai a iʻole lyophilized paha ma hope o ka heluʻana ma hope o 2 mau pule inā mālamaʻia ma 2 - ℃.

Eʻoluʻolu e pale i nā manuahi.

Eʻoluʻolu e kelepona iā mākou no kekahi hopohopo.