Il - 6 - Mab │ mose Antti - Kōkuhi inoa

Nā Hōʻoia Pūnaewele:

Il - 6 kahi cytokine nui i pili i ka regulate immune, ka hoʻouluʻana, a me nā kaʻina hana pilikino, a me nā kumuʻelua a anti - nā hopena hopena. ʻO kāna kuleana i loko o ka paneʻana i ka maʻi nui, acute - Nā hopena maikaʻi loa, a hiki i keʻano he mea nānā i kahi mea hana ma ka maʻi maʻi.

Molecularʻano:

ʻO ka Antibody Antibody i heluʻiaʻo MW o 160 Kda.

Nā noi noi:

Ho'ōlaʻo hope i IMMANOOAY, ELisa

Ua'ōleloʻia:

ʻO ka noi no ka double -ʻO Atibody Sandwich no kaʻike,ʻelua me mi03702 no ka hopuʻana.

'Ōnaehana Bufer:

0 0 0Mha PB, pH7.4

Respsons:

Eʻoluʻolu eʻike i ka palapala hōʻoia o ka loiloi (COA) no ka hoʻounaʻiaʻana me nā huahana.

Ka Hoʻoulu:

ʻO ka Antibody ma kahi o keʻano wai i laweʻia ma ka puka brozen me ka hau polū.

Huanui:

No ka hoʻolimalima lōʻihi, ua paʻa ka huahana no nā makahikiʻelua e mālamaʻia ma - 20 ℃ a haʻahaʻa paha.

Eʻoluʻolu e hoʻohana i ka huahana (ʻano wai) i loko o 2 mau pule inā mālamaʻia ma 2 - 0 ℃.

Eʻoluʻolu e pale i nā manuahi.

Eʻoluʻolu e kelepona iā mākou no kekahi hopohopo.