Cov Tshuaj Tiv Thaiv Kab Mob Ntaus Hom Tshuaj Tiv Thaiv Tshuaj Ntsuam (Elisa)

Txheej txheem assay

Kauj ruam 1: Tus lej
Kauj ruam 2: Npaj qauv
Kauj ruam 3: ncu
Kauj ruam 4: Ua kua roj teeb tsa
Kauj ruam 5: Ntxuav
Kauj ruam 6: Ntxiv enzyme
Kauj ruam 7: ncu
Kauj ruam 8: Ntxuav
Kauj ruam 9: Xim
Kauj ruam 10: Nres cov tshuaj tiv thaiv
Kauj ruam 11: laij

Cov Khoom Qhia:

Cov khoom siv yog rau kev txiav txim siab zoo ntawm IBV AB hauv cov qauv, tau khoom ib qho khoom siv lub ntsej muag lossis cov qauv pipette rau cov qhov dej, nrog anti - IBV AB conjugated horseradish peroxidase (hrp). Ntxuav thiab tshem tawm cov tsis - sib txuas hlau tshuaj thiab lwm yam. Antibodies tshwj xeeb rau cov antigen yuav khi rau lub ntej - coated antigen. Tom qab ntxuav tag nrho, ntxiv tmb substrate daws thiab cov xim tsim raws li tus nqi ntawm IBV AB. Cov tshuaj tiv thaiv yog kev txiav tawm los ntawm kev sib ntxiv ntawm ib qho kev sib ntxiv thiab kev siv ntawm cov xim yog ntsuas ntawm ib qho yoj ntawm 450 nm. Piv nrog lub cutoff tus nqi los txiav txim yog tias ib tug abv abs tshwm sim hauv cov qauv lossis tsis.

Daim ntawv thov:

Cov khoom siv xeem tso cai rau kev txiav txim siab ntawm tus kab mob sib kis mob tshuaj tiv thaiv kab mob bouldody thiab ntshav, tuaj yeem siv cov tshuaj tsw qab thiab ntshav paug, tuaj yeem siv cov tshuaj tiv thaiv kev txhaj tshuaj tiv thaiv kab mob rau cov tshuaj txhaj tshuaj tiv thaiv kab mob rau kev txhaj tshuaj tiv thaiv kab mob txhaj tshuaj.

Cia: Khaws rau ntawm 2 - 8 ℃ thiab zam kom tsis txhob ntxwm.

Cov Qauv Thawj Coj:Tus qauv thoob ntiaj teb.