Methylated Septin 9 Nplooj Pr Cov Khoom Siv Tau

Khoom Nqe lus piav qhia:

Septin9 thiab Ndrg4 noob caj tswv methylation yog ib lub sijhawm tiag tiag - PCRAsy DNA Methylation hauv Cell dawb rho tawm los ntawm tib neeg ntshav. Cov khoom siv no yog ua raws Taqmantm DNA polymerase amplification amplification thiab fluorocrome - sau npe tshwj xeeb kev sojntsuam. Tom qab bisulfite hloov dua siab tshiab ntawm unmething cytosine nucleotides rau uracil ntawm septin9 thiab cov cuab yeej siv sab hauv, DNA rho tawm, thiab kev rho tawm.

 
Lwm Cov Khoom Yuav Tsum Tau:

1.fluorescence PCR intable ntawm kev nyeem ntawv tshaib plab (494nm Ntau tshaj nqus, 518nm Qhov siab tshaj plaws) thiab Joe (545nm Qhov siab tshaj plaws). Nco tseg: Vic channel tuaj yeem siv tau nyob rau hauv qhov chaw ntawm Joe ntawm cov twj paj nruag uas Joe tsis tau ntsuas.

2.Vortex tov khoom

3.MicRocentrifug pom

4.Pipettes

5.Sterile Nuclease - Free Pipette Cov Lus Qhia (Cov Lus Qhia Barrier Cov Lus Qhia kom pom zoo) thiab cov raj microfuge

6.PRICE VCR Phaj

 Daim Ntawv Thov:

Cov khoom siv no yog siv rau qhov kev ua tau zoo hauv cov methylated septin9 (MST9) thiab ndrg4 cov noob hauv cell dawb rho tawm los ntawm tib neeg ntshav.

Cia: Cov khoom siv tuaj yeem khaws cia ntawm - 20 ° C rau lub sijhawm 12 lub hlis ua ntej qhib. Tom qab qhib, cov reagents yog siv tau tsawg kawg 6 lub hlis yog muab khaws cia ntawm - 20 ° C.

Cov Qauv Thawj Coj:Tus qauv thoob ntiaj teb.