Newcastle Kab Mob Tshuaj Tiv Thaiv Elisa Cov Khoom Siv
Cov Khoom Qhia:
Cov khoom siv kab mob tshiab cov tshuaj tiv thaiv Elisa Cov Khoom Siv yog cov cuab yeej ntawm cov tshuaj tiv thaiv kab mob tshwj xeeb (NDV) hauv Ntshav Qab Zib lossis Plasma kuaj los ntawm nqaij qaib. Siv cov enzyme - txuas immunosorbent assay (Elisa) cov txheej txheem, cov khoom siv no muaj cov kab ke rhiab thiab cov kab mob uas tau raug rau cov kab mob. Cov khoom siv tau xws li tag nrho cov khoom siv uas tsim nyog, xws li ua ntej antigens, kev tswj hwm, thiab kev kuaj xyuas kom muaj txiaj ntsig thiab raug hauv kev kuaj.
Lub hauv paus ntsiab lus ntawm kev xeem:
Cov khoom siv no siv Block Elisa txoj kev, NDV antigen yog pre - coated ntawm microplate. Thaum kuaj, ntxiv diluted serum qauv, tom qab tsim kom loj, nws yuav sib xyaw ua ke nrog cov tshuaj tiv thaiv antigodys, pov tseg cov tshuaj tiv thaiv antomody thiab lwm yam nrog kev ntxuav; Tom qab ntawd ntxiv enzyme Labled anti - NDV monoclonal antibody, antibody hauv cov qauv block ntawm monoclonal antibody thiab pre - coated antigen; Muab pov tseg cov enzyme unleonme conjugate nrog ntxuav. Ntxiv tmb substrate nyob rau hauv micro - Wells, lub cim xiav xiav los ntawm enzyme catalysis muaj intibody cov ntsiab lus hauv cov qauv.
Daim ntawv thov:
Kev kuaj pom ntawm cov tshuaj tiv thaiv tshwj xeeb ntawm Newcastle Reteaspecific Antibodies
Cia:Txhua tus reagents yuav tsum muab cia ntawm 2 ~ 8 ℃. Tsis khov.
Cov Qauv Thawj Coj:Tus qauv thoob ntiaj teb.
Txheem:
|
Reagent |
Ntim 96 Kev Ntsuam Xyuas / 192Tests |
1 |
Antigen Coated Microplate |
1ea / 2, 2, 2 |
2 |
Kev tswj hwm tsis zoo |
2ml |
3 |
Kev Tswj Xyuas Zoo |
1.6ml |
4 |
Qauv rau mob |
100ML |
5 |
Ntxuav cov tshuaj (10xconcentrated) |
100ML |
6 |
Enzyme conjugate |
11 / 22ml |
7 |
Substrate |
11 / 22ml |
8 |
Nres kev daws teeb meem |
15ML |
9 |
Nplaum phaj foob |
2,enea |
10 |
Ntshav Dilution Microplate |
1ea / 2, 2, 2 |
11 |
Kev qhia |
1 pcs |