Porcine Pseudorabies mob GD (PRV - Gd) Antibody Elisa Khoom siv

Cov Khoom Qhia:

Cov khoom siv no muaj ib lub microtiter phaj nrog PRV - Gd, enzyme conjugates, thiab lwm yam nrog reagents. Nws ua haujlwm lub hauv paus ntsiab lus ntawm enzyme - txuas immunosorbent assay (Elisa) los kuaj cov tshuaj tiv thaiv prv - GD hauv cov ntshav ntshav lossis ntshav. Thaum lub sij hawm sim, tswj cov ntshav dej thiab cov qauv ntsuas yog ntxiv rau lub phaj. Tom qab tsim kom loj, yog tias tus qauv muaj prv - GD tiv thaiv, lawv yuav khi rau cov antigens coated ntawm lub microtiter. Cov kauj ruam ntxuav ua raws li los tshem tawm cov txheej txheem tsis hloov pauv, cov enzyme conjugates yog ntxiv, uas khi tshwj xeeb rau cov antigen - antibody complexes ntawm lub phaj. Tom qab ntxuav rov qab tshem tawm cov tsis muaj kev sib xyaw ua ke, substrate reagents yog ntxiv rau cov dej thiab hnov ​​nrog cov enzyme - cov xim xiav, ua rau muaj xim xiav. Qhov kev siv ntawm cov xim yog ncaj qha rau cov nyiaj ntawm cov tshuaj tiv thaiv tshwj xeeb hauv cov qauv. Cov tshuaj tiv thaiv yog tom qab ntawd tshem tawm los ntawm kev ntxiv cov tshuaj nres nres, tig cov daws teeb meem daj. Kev nqus tau ntawm txhua qhov zoo yog ntsuas ntawm lub wavelength ntawm 450nm siv tus nyeem ntawv phaj microtriter (micrpleupate nyeem ntawv) los txiav txim qhov muaj ntawm PRV - GD Tshuaj tiv thaiv kab mob.

Daim ntawv thov:

Qhov zoo no tau tsim los tshawb nrhiav cov tshuaj tiv thaiv pseudorabies mob glycoprotein B (PrV - GD) hauv Porcine ntshav lossis ntshav. Nws tuaj yeem siv rau kev ntsuam xyuas ntawm PSEUDORRICATION ntawm Pseudorabies tus kab mob hauv npua.

Cia: 2 - 8 ° C

Cov Qauv Thawj Coj:Tus qauv thoob ntiaj teb.