Porcine Toxoplasma Gondi Kev Ntsuas Cov Khoom Siv (Elisa)

Kev sib xyaw ntawm cov khoom siv:

Microeliy sipplate, hrp - conjugate reagent, chromogen daws b, nres kev daws teeb meem, kev tswj hwm zoo, tsis zoo tswj hwm.

Lub ntsiab cai ntawm kev xeem:

Cov khoom siv yog rau kev txiav txim siab zoo ntawm tox ab hauv cov qauv, ua kom tawv antigen rau lub tsho ntev Porcine tox abs conjugated horseradish peroxidase (hrp). Ntxuav thiab tshem tawm cov tsis - sib txuas hlau tshuaj thiab lwm yam. Antibodies tshwj xeeb rau cov antigen yuav khi rau lub ntej - coated antigen. Tom qab ntxuav tag nrho, ntxiv tmb substrate daws thiab xim tsim raws li tus nqi ntawm tox ab. Cov tshuaj tiv thaiv yog kev txiav tawm los ntawm kev sib ntxiv ntawm ib qho kev sib ntxiv thiab kev siv ntawm cov xim yog ntsuas ntawm ib qho yoj ntawm 450 nm. Piv nrog rau kev txiav cov nqi txiav txim siab yog tias tox abs tshwm sim hauv cov qauv lossis tsis.

Cov Khoom Qhia:

Cov khoom siv xeem tso cai rau kev txiav txim siab ntawm Porcine Toxoplasma antibodasma antibodasma (tox - AB) Qhov kev txhaj tshuaj tiv thaiv kab mob toxopine TocopineMas, tuaj yeem siv rau cov tshuaj tiv thaiv kev txhaj tshuaj rau cov tshuaj tiv thaiv toxopine

Ntsuas: Mircoplate nyeem ntawv (muaj: 450nm, 630nm wavelength), cov cua nab kuab (37 degrees Celsius), kho tau pipette.

Cia: Cov khoom siv yuav tsum tau khaws cia rau ntawm [2 - 8 ℃]. Qhov qhib microelisa sipplate tuaj yeem khaws cia ntawm [2 - 8 ℃] thiab zam kom tsis txhob ntxwm. Siv tsawg kawg 2 lub hlis.

Cov Qauv Thawj Coj:Tus qauv thoob ntiaj teb.