Kitcoplasma Gallopsomum AB (Elisa)

Usoro Elisa:

1) Were akwukwo (enwere ike itinye ego na-ejikarị ọtụtụ oge iji tụọ ọfụma), tinye 100μl dig 1 maka nchịkwa na-adịghị mma, 2 nke ọma maka ikike na-adịghị mma. Tinye 100μl na-adịghị mma / na-achịkwa olulu mmiri ya. Jiri nwayọ, egbuola, kpuchie ya ma tinye ya na 37 ℃ maka 30 min.

2) Wụsa mmiri mmiri ahụ n'ime olulu mmiri, tinye 250 μl long na-asacha na-asa ahụ nke ọ bụla, wụsa. Tinyegharịa 4 - 6 ugboro, na pat ikpeazụ iji kpọọ nkụ.

3) Tinye 100μl enzyme conjugate nke ọma, maa jijiji, kpuchie ya na 37 ℃ maka 30 min.

4) Tinyegharịa nzọụkwụ 2 (ịsa). Cheta na pat kporo nkụ na-atọ ụtọ na ikpeazụ.

5) Tinye 100μl mkpụrụ na nke ọ bụla, gwakọta nke ọma, meghachi omume maka 10 min Atdark na37 ℃ On.

6) Tinye 50μL nke nkwụsị nkwụsị na nke ọ bụla, wee tụọ nsonaazụ ahụ n'ime 10 min.

Nkọwa nke ngwaahịa:

Ebumnuche Mycoplasma (MG) Ansanod Alisa Kit sitere na immuniasay na-adịghị mma (na-ezighi ezi Elisa). Mgbe otu esemokwu ahụ nwere ọgwụ mgbochi megide nje, ha ga-ekekọta antigen na efere. Sachaa ọgwụ mgbochi na ihe ndị ọzọ. Tinyezie otu enzyme conjugate. Mgbe incubation na ịsacha, gbakwunye mkpụrụ TMB. Mmeghachi omume na-acha odo odo ga-apụta, ọ na-atụle site na ụdị dị mma (450 nm).

Akwukwo icho oru:

A na-eji Kit a iji chọpụta Galloplasma (MG) Antirodma na ọkụkọ akwara nke Mycoplasma na ugbo ọkụkọ na-enyere aka na ọkụkọ Seracal.

Nchekwa: Ịchekwa na 2 - 8 ℃ n'ọchịchịrị.

Ụkpụrụ ndị isi:International Station.