Methylated Septin 9 PCR Detection Kit

Methylated Septin 9 PCR Detection Kit

Short Description:

Common Name: Methylated Septin 9 PCR Detection Kit

Category: Point of Care Test (POCT)-Molecular Diagnostic Test

Test Sample: DNA

Specificity: No cross reactivity was identified when the kit was evaluated.

Brand Name: Colorcom

Shelf Life: 12 Months

Place of Origin: China

Product Specification: 96T


Product Detail

Product Tags

Product Description:

Septin9 and NDRG4 gene methylation detection kit is a real-time PCR assay for the detection of abnormal DNA methylation in cell free DNA extracted from human plasma. This kit is based on TaqmanTM DNA polymerase amplification and fluorochrome-labeled specific probe reporting. Following bisulfite conversion of unmethylated cytosine nucleotides to uracil, this kit detects methylation of septin9 and NDRG4 genes along with an internal control, actb to monitor sample collection, DNA extraction, and amplification.

 
Other Materials Required:

1.Fluorescence PCR instrument capable of reading FAM (494nm maximum absorption, 518nm maximum emission) and JOE (520nm maximum absorption, 545nm maximum emission). Note: VIC channel can be used in place of JOE on instruments for which JOE has not been calibrated.

2.Vortex Mixer

3.Microcentrifuge

4.Pipettes

5.Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes

6.Compatible PCR Plate

 Application：

This kit is used for the qualitative in vitro detection of methylated SEPTIN9 (mSEPT9) and NDRG4 genes in cell free DNA extracted from human plasma.

Storage: The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.

Executive Standards: International Standard.

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