Methylation Septin 9 PCR Whakamatau

Hua Whakaahuatanga:

Septin9 me te Ndrg4 ira kohukohu METHURCHUS he tino - I te wa e kii ana a PCr mo te kitenga o te methylation DNA Ko tenei kete kei runga i te Taqmantm DNA Polymeralease Aptification me te fluorochrome - tapaina te ripoata a-tuhi mo te tohu. Whai muri i te hurihanga o Bisulfrite o te Cytosine Nucleotides ki Uracil, ka kitea e tenei kete te kohukohu o te Septin9 me te NDRG4 me te mana whakahaere, me te whakanui.

 
Ko etahi atu taonga e hiahiatia ana:

1.Forescence PCR PCR e taea ana te panui mo te whanau (494nm toputanga nui rawa atu, 518nm te nui o te kohinga) me te joe (520nm toputanga nui, 545nm te nui o te whakakoretanga). FAKATOKANGA: Ka taea te whakamahi i te hongere VIC ki te waahi o Joe i runga i nga taonga mo te mea kaore i te whakahekehia a Joe.

2.Vortex whakaranu

3.microcentrifuge

4.Pipette

5.Maile Nugase - He Tohu Pipette Free (tohutohu arai i tūtohutia) me nga ngongo microfuge

6 pātene pcr pcr

 Tono:

Ka whakamahia tenei kete mo te taatai ​​i roto i te tohu vitro o te septin9 (MSEPT9) me te NDRG4 ira i roto i te punaha koreutu.

Rokiroki: Ka taea te penapena i te kete i - 20 ° C mo te waa 12 marama i mua i te whakatuwheratanga. I muri i te whakatuwheratanga, ka whakamanahia nga reagents mo te 6 marama ki te penapena i te 20 ° C.

Paerewa whakahaere:Paerewa International.