Mycoplasma gallisepticum Ab Test Kit (ELISA)

ELISA procedure:

1) Take pre-coated microplate (Can unseal for several time use as per sample quantity), add 100μL diluted serum to sample wells, meanwhile set 1 well for Negative control, 2 wells for Positive control separately. Add 100μL Negative/Positive control to its wells. Shake softly, do not overflow, cover and incubate at 37℃ for 30 min.

2) Pour the liquid out of the wells, add 250 μL diluted washing buffer to each well, pour out. Repeat 4-6 times, at last pat to dry on absorbent paper.

3) Add 100μL Enzyme conjugate to each well, shake softly, cover andincubate at 37℃ for 30 min.

4) Repeat the step 2(washing). Remember pat to dry on absorbent paper at last.

5) Add 100μL substrate to each well, mix properly,react for 10 min atdark at37℃ in dark.

6) Add 50μl of stop solution in each well, and measure the result within 10 min.

Product Description:

The Mycoplasma Gallisepticum(MG) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).

Application:

This kit is used to detect Mycoplasma Gallisepticum(MG) antibody in chicken serum, to assess antibody condition by Mycoplasma Gallisepticum(MG) vaccine in chicken farm and assist diagnosis of serological infected chicken.

Storage: Storing at 2-8℃ in the dark.

Executive Standards: International Standard.