Haemophis parasuis kuyesa kép (RT - PCR)
Kutanthauzira kwa Zogulitsa:
Ichabwino |
Nchipinda |
Khumi - Bokosi La Bokosi la Boy |
2 × qpcr amatsenga |
90408 |
500μL (Brown Cap) |
A Fluents a PCR Templates |
180701 |
1 ml (chipewa chachikaso) |
Pcr primer osakaniza a bovine herpesvirus |
14 - 51800yw |
100μL (chipewa choyera) |
Kuwongolera koyenera kwa bovine herpesviruvis pcr (1 × 10e8 makope / μl) |
14 - 51800pc |
50μl (chipewa chofiira) |
Viral lysis lysis ya DNA Virus (paketi yoyeserera) |
367A |
Nthawi 15 (9ml) |
Mafotokozedwe Akatundu:
Izi zikuyenera kusintha zomwe zimachitika pakusintha kwa ma RNA ndi zotsatira za PCR zomwe zimakwezedwa. Imagwiritsa ntchito m - MLV Potengera zomwe zinasinthiratu, kupangitsa mbadwo wazinthu zazitali za CDNA. Kuphatikiza apo, mu 20 μl chosakanikirana cha 20 μL ndi kuchuluka kwa ma 50. Ma enzymes omwe akuphatikizidwa m'magulu awa atumizidwa, ndi rt enzyme kukhala mlzme yotumizidwa ndi - mlv, zomwe zimapangitsa kuti CDA ikhale ikuluitali ndikusunga kwathunthu kwa zidziwitso za gene! Mphongo yoletsa kugwiritsidwa ntchito panthawi yosinthira kusinthidwe imachepetsa chiopsezo choyesera kulephera chifukwa cha kuipitsidwa kwa RNASE. Izi ndizosavuta komanso kugwiritsa ntchito mwachangu, zomwe zimapangitsa kuti zikhale zopota za CDNA
Karata yanchito:
Izi ndizosavuta komanso kugwiritsa ntchito mwachangu, zomwe zimapangitsa kuti zikhale zopota za CDNA
Kusungira: Sungani 20 ° C. Pewani Kupuma Kwambiri - Thaw Cycles.
Mfundo Zapamwamba:Muyezo wapadziko lonse lapansi.