Methylated septin 9 pcr yozindikira zida
Chinthu Kufotokozera:
Septin9 ndi NDGEMEEMETETE ITEYLUSTE YOPHUNZITSIRA DCR PCR Gantany ya Kuzindikira kwa Alemer DNAMPT DNA Free Free DNA. Kityi yachokera ku Taqmantm DNA Polymeseset Kukula ndi Fluorochrome - Pulogalamu Yodziwika Yolemba. Kutsatira ma bipulfite kutembenuka kwa cytosine ya ma cytosine ku Uraclines, izi zimazindikira mesylation ya septin 2 ndi ntransi ya mkati ndi kuwunikira zitsanzo, DNA yowonjezera.
Zida zina zofunika:
1.Forescence PCR CCR yomwe imatha kuwerenga banja (494nm kwambiri, 518nm Express) ndi a Joe (520nm mayamwidwe ambiri, 545nm Express Exporse). Dziwani: Njira ya vic imatha kugwiritsidwa ntchito m'malo mwa joe pazida zomwe Joe sizinafalitsidwe.
2.Vortex chosakira
3.Mimrocentrifigege
4.Pipette
5.SsSSST NACKECE - Malangizo aubwenzi a Pipe
6.COMPEGE PCR Pulala
Ntchito:
Kityi imagwiritsidwa ntchito pozindikira ku Vitated ku methylated Sepport Septor9 (Mbep9) ndi majini a NDGE4 mu cell Free DNA.
Kusungira: Kit ikhoza kusungidwa ku - 20 ° C kwa miyezi 12 isanakwane. Mukatsegulira, ma reagents ndi othandiza kwa miyezi isanu ndi umodzi ngati yosungidwa ku - 20 ° C.
Mfundo Zapamwamba:Muyezo wapadziko lonse lapansi.