Mycoplasma gallisepticulam al poyesa (Elisa)

Machitidwe a Elisa:

1) Imwani Precroplate ya Preroproplate (ikhoza kusagwiritsidwa ntchito kwakanthawi kochepa), onjezerani 100μL. Pakadali pano Onjezani ma 100μL osayenera kuwongolera zitsime zake. Gwedezani modekha, osasefukira, chivundikiro ndi kulumikizana pa 37 ℃ kwa mphindi 30.

2) Thirani madzi pachitsime, onjezerani 250 μl yothira kuchapa pachitsime chilichonse, kutsanulira. Bwerezani 4 - maulendo 6, pomaliza pat kuti muume pa pepala lotakamwa.

3) Onjezani ma enzyme a newjme conjuguate pachitsime chilichonse, chitani modekha, chivundikirani IndiCubate pa 37 ℃ kwa 30 min.

4) Bwerezani Gawo 2 (kutsuka). Kumbukirani pa pat kuti muume pa pepala lotakataliraliza.

5) Onjezani 25μl gawo lililonse pachitsime, sakanizani bwino, gwiritsani 10 Min Atdark at37 ℃ mdima.

6) Onjezani 50μl ya kuyimitsa yankho lililonse pachitsime chilichonse, ndikuyeza zotsatira zosakwana 10 min.

Mafotokozedwe Akatundu:

Mycoplasma gallisepticulam (mg) antibody elsissa Kit imakhazikitsidwa pa enzymact imzymasay (mwachindunji Elisa). Antigen amapezeka pa mbale. Chiwonetsero cha zitsanzo chikakhala ndi ma antibodies apadera motsutsana ndi kachilombo, adzamanga ku Antigen pa mbale. Sambani ma antibodies osasunthika ndi zina zophatikizira. Kenako onjezani enzyme inanso yolumikizirana. Pambuyo makulitsidwe ndikutsuka, onjezerani TMB gawo. Ndondomeko ya utoto imawoneka, yoyesedwa ndi mawonekedwe a Spectophotometer (450 nm).

Karata yanchito:

Kityi imagwiritsidwa ntchito kuwona mycoplasmma gallisepticulam (mg) anti-anti-ku nkhuku seramu, mg) katemera wa nkhuku ndikuthandizira kuzindikira nkhuku yomwe ili ndi serological.

Kusungira: Kusunga pa 2 - 8 ℃ mumdima.

Mfundo Zapamwamba:Muyezo wapadziko lonse lapansi.