Porcine Pseudorabies Virus gD (PRV-gD) Antibody ELISA Kit

Product Description:

This kit comprises a coated Microtiter Plate with PRV-gD, enzyme conjugates, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against PRV-gD in the porcine serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains PRV-gD antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound enzyme conjugates, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of PRV-gD antibodies  in the sample.

Application:

This assay is designed to detect antibodies against pseudorabies virus glycoprotein B (PRV-gD) in porcine serum or plasma. It can be used for the evaluation of the immunological efficacy of the pseudorabies virus vaccine in pigs.

Storage: 2-8°C

Executive Standards: International Standard.