Methylated Septin 9 PCR itererana ibikoresho

Ibicuruzwa Ibisobanuro:

Septin9 na NDRG4 Gen Methylation Kit ni umuntu nyawe - Umwagihe PCR wasabye uburyo bwo kumenya imigati idahwitse ya ADN yakomokaga kuri Plasma yabantu. Ibi bikoresho bishingiye kuri taqmantm dna polymerase yoroshya na fluorochrome - yanditseho raporo yihariye. Gukurikira ihinduka rya BisulfIte rya Ctosine ya Ctosine ya Ctosine kuri Ural, iyi Kit itanya imigereka ya serivili ya Septrin9 hamwe no kugenzura imbere, Acb gukurikirana icyegeranyo cyicyitegererezo, gukuramo ADN, no kongera.

 
Ibindi bikoresho bisabwa:

1.Fluorezecence PCR ishoboye gusoma umuryango (494nm ntarengwa, 518nm imyuka ntarengwa) na joe (520nm ikoresheje ntarengwa, 545nm imyuka ntarengwa). Icyitonderwa: Umuyoboro wa VIC urashobora gukoreshwa mu mwanya wa Joe kubikoresho joe bitarahinduwe.

2.vortex mixer

3.microcentRuge

4.pipettes

#Iclease - Inama za pipette yubusa (interineti za bariyeri zirasabwa) na microfuge tubes

6.Icyiciro cya PCR

 Gusaba:

Iyi kin ikoreshwa mubyifuzo muri Vitro Kumenya Fitro ya ETHYYSTS Septhin9 (Msept9) na jan ya NDRG4 kuri selile kubuntu dNA ikomoka kuri plasma yumuntu.

Ububiko: Ibikoresho birashobora kubikwa kuri - 20 ° C mugihe cyamezi 12 mbere yo gufungura. Nyuma yo gufungura, abasubiramo bafite byibuze amezi 6 niba babitswe kuri - 20 ° C.

Ibipimo ngenderwaho:Amahame mpuzamahanga.