Mycoplasma gallisepticum ab ikizamini (Elisa)

Uburyo bwa Elisa:

1) Fata imbaho ​​- Microplate (irashobora gukoreshwa igihe kinini nkuko byikubye ingano) Ongeramo 100μl nabi / kugenzura neza amariba yayo. Shake gahoro, ntukarengere, upfundikire kandi ushizwe kuri 37 ℃ iminota 30.

2) Suka amazi mu mariba, ongeraho 250 μl zatandukanije buffer yo gukaraba kuri buri ndunduro, suka. Subiramo 4 - inshuro 6, pat ya nyuma yo gukama kumpapuro zinjira.

3) Ongeraho 100μl enzyme guhuza kuri buri shyanga, shake buhoro, gutwikira hamwe na 37 ℃ iminota 30.

4) Subiramo Intambwe ya 2 (Gukaraba). Ibuka Pat kugirango byume impapuro zinjira amaherezo.

5) ongeraho 100μl substrate kuri buri shyanga, vanga neza, witondere kuri 10 min atdark kuri37 ℃ mu mwijima.

6) Ongeraho 50μl yo guhagarika igisubizo muri buri cyenda, hanyuma upime ibisubizo muminota 10.

Ibisobanuro by'ibicuruzwa:

Mycoplasma galliseptum (mg) antibody Elisa kit ashingiye ku kuri enzymass ya enzymatic Iyo icyitegererezo Serusi arimo antibodies yihariye kurwanya virusi, bazahambira antigen kumasahani. Oza antibodies zitara rirenze nibindi bigize. Noneho ongeraho enzyme yihariye. Nyuma yo guhagarika no gukaraba, ongeraho TMB. Igitekerezo cyamabara kizagaragara, gipimwa na spectraphotometero (450 nm).

Gusaba:

Iyi Kit ikoreshwa mugutangiza mamaiseplasma gallisepticum (mg) antibods muri sarum yinkoko, kugirango isuzume imiterere ya Mycoplasma Gallisepma (MG) urukingo rwumurima winkoko no gufasha gusuzuma inkoko yanduye ikariso.

Ububiko: Kubika kuri 2 - 8 ℃ mu mwijima.

Ibipimo ngenderwaho:Amahame mpuzamahanga.