Nooca fayraska cagaha iyo afka ee cudurka loo yaqaan 'asiaⅰab'

Sharraxaadda Wax soo saarka:

Qalabkan waxaa ka kooban HRP conjugate, dib-u-habaynta kale ee saliidda iyo saxanka Elisa microtate plate pre - ku dahaarka leh noocyada vidas-ka lugta iyo afka ee 'FMD - Aslia) borotiinka. Codso mabda'a enzyme - Lasthossassay (Elisa) si loo ogaado Porcine FMD - Cayridka ka hortagga muunadaha doofaarka ee swiness. Intii lagu gudajiray tijaabada, ku dar xakameynta xamuulka iyo saamballada saxanka Elisa microttiter. Haddii FMD - Astaan ​​difaac ka hortag ah oo ka mid ah samballada, waxay ku xidhan tahay dib-u-dejinta proteinka dib-u-dejinta ee saxanka microtitter ka dib. Ka dib waxaad u rajeyneysaa saxanka si ay uga saarto unugyada unugyada aan tooska ahayn iyo waxyaabaha kale, ku dar HRP consjugate si ay si gaar ah ugu xidhaan xarunta antibody iyo antigen saxanka weelka microtite. HRP-ga aan loo baahnayn oo la isku halleeyo ayaa laga saari doonaa dhaqitaanka. Substrate-ka ayaa lagu soo kordhiyaa sidoo kale, waxay ka falcelin doontaa enzyme iyo soo-saareyaashu waxay noqon doonaan buluug. Shaashadda midabka ayaa ah in isqarxin wanaagsan oo leh heerarka ka hortagga sanballada. Ugu dambeyntiina, ku soo afjaro fal-celinta adoo ku daraya xalka joogsiga si loo soo saaro wax soo saar jaale ah. Cabbir qiimaha u nugul ee well kasta adoo adeegsanaya akhristaha microtitter-ka microtitter-ka leh 450 nm wavoththth, ka dib waxaan ogaan karnaa inay jiraan Boorso FMD - Cayridka FMD ee samballada.

Isticmaal:

Nooca cagaha iyo afka Cudurka Cudurka Cudurka ASIA ⅰ Qalabka Tijaabada (Elisa) waxaa loo isticmaalaa ogaanshaha unugyada ka hortagga ah ee nooca fayraska cudurrada ee cagta ee loo yaqaan 'Asia' ee loo yaqaan 'plasma' ama tusaalooyinka plasma.

Kaydinta:Dhammaan reages-ka oo dhami waa in lagu kaydiyaa 2 ~ 8 ℃. Ha qaboojin.

Heerarka Fulinta:Heerka caalamiga ah.