Qalabka tijaabada ee haemophilus parazilus (rt - pcr)
XUQUUQDA ASHIIQADA:
Ingrug |
NUmber |
Toban - xirmo sanduuqa god |
2 × Qpr Magicmix |
90408 |
500μl (CAP Brown) |
Diyaaradii Foorsest PCL Tunplates |
180701 |
1 ml (cap casule) |
PCR isku-darka pr-ka ee loo yaqaan 'povine herpesviras' |
14 - 51800yw |
100μl (dabool cad) |
Xakamaynta togan ee bovine Herpesviras pcr (1 × 10e8 koobiyo / μl) |
14 - 51800pc |
50μl (cap cap) |
Xalka viral viasis ee fayraska DNA (xirmada tijaabada) |
3674a |
15 times (9mL) |
Sharraxaadda Wax soo saarka:
Xirmadan ayaa ku habboon in jawaab-celinta la wareego ee muunadaha kala duwan ee RNA iyo cod-weynaha xiga ee PCR. Waxay isticmaashaa m - MLV ee jawaab celinta dib-u-celinta, oo u saamaxaya jiilka wax soo saarka CDNA dheer. Intaa waxaa sii dheer, in 20 μl resfedero isku darka lagu daro iyo mugga 50 μl pcr pcr, waxay bixisaa tiro ku filan oo ah badeecada PCR ee tijaabooyinka hoose ee kabaha hoose. Enzymes-ka lagu soo daro xirmadan ayaa la keenay, iyadoo Rt enzyme uu yahay mid la soo dejiyo m Xaraashka qaaska ah ee loo adeegsaday inta lagu gudajiray inta lagu gudajiro hanaanka la qorsheeyay ee waxtarka leh wuxuu si wax ku ool ah u yareeyaa halista ku-guuldaradii tijaabada ah ee tijaabada ah sababtuna tahay faddaraynta excoge. Qalabkan waa mid sahlan oo deg deg ah in la isticmaalo, taasoo si balaadhan u khuseyso si ay ugu dabaqaadato cdna clonking iyo bartilmaameedka ogaanshaha hiddaha ee ogaanshaha cilmiga barashada cilmiga bayoolajiga ee molotalaal.
Isticmaal:
Qalabkan waa mid sahlan oo deg deg ah in la isticmaalo, taasoo si balaadhan u khuseyso si ay ugu dabaqaadato cdna clonking iyo bartilmaameedka ogaanshaha hiddaha ee ogaanshaha cilmiga barashada cilmiga bayoolajiga ee molotalaal.
Kaydinta: Ku keydi 20 ° C. Ka fogow qaboojiyaha soo noqnoqda - wareegga tuubada.
Heerarka Fulinta:Heerka caalamiga ah.