Mycoplasma Gallopticum Gallopticum AB Kit (Elisa)

Nidaamka Elisa:

1) Qaado pre - microplate-ka qunyar-galeenka (waxay ku fidin kartaa dhowr waqti oo loo isticmaalo tiro kasta oo muunad ah), ku dar 100μl Serum-ka oo muunad ah, oo si fiican u dejiso si wanaagsan oo si fiican u xakameeya. Ku dar 100μl taban / kontorool togan ceelasha. Si tartiib ah u rux, ha daboolin, daboolin hana ku biirin 37 ℃ 30 daqiiqo.

2) Dareeraha ka soo baxa ceelasha, ku dar 250 μL wax lagu dhaqo wax lagu dhaqo si wanaagsan, ku shub. Ku celi 4 - 6 jeer, oo ah Pat Pat si aad u qalajiso warqad galka ah.

3) Ku dar 1005μl enzyme conjugate si fiican, rux si tartiib ah u rux, dabool oo 37 ℃ 30 daqiiqo.

4) Ku celi tallaabada 2 (maydhista). Xusuusnow Pat si aad u qalajiso warqad galka ah ugu dambeyntii.

5) Ku dar 1005μl subsstrate mid walba, si fiican isugu qas, ka falceli 10 Min Atdak At 37 ℃ mugdi.

6) Ku dar 50μl xalka joogsiga si wanaagsan, oo cabir natiijada 10 daqiiqo gudahood.

Sharraxaadda Wax soo saarka:

Galletlasma Gallepticum (MG) Qalabka antibody elsody elesatic wuxuu ku saleysan yahay enzysusass enzysusass-ka (ELISET ELISA) Marka muunad serum ay ka kooban tahay unugyo ka hortag ah fayras, waxay ku xidhi doonaan antigen taarikada. Ku dhaq baaritaanka jirka ee aan la soo koobi karin iyo qaybaha kale. Ka dib ku dar enzyme gaar ah oo la isku daro. Ka dib markii la soo daro iyo dhaqitaanka, ku dar qaybta 'TMB'. Falcelinta midabka ayaa soo muuqan doonta, oo lagu cabiraa muuqaalka spectophotometer (450 NM).

Isticmaal:

Qalabkan waxaa loo isticmaalaa in lagu ogaado Mycoplasma Gallsepticum (MG) Daawooyinka digaagga, si uu u qiimeeyo xaaladda ka hortaga mycoplasma glasepicicum (MG) ee beerta digaagga iyo caawinta ogaanshaha digaagga cudurka ee nafaqada leh.

Kaydinta: Kaydinta 2 - 8 ℃ mugdiga.

Heerarka Fulinta:Heerka caalamiga ah.