Cudurka 'Newcastle' Antisody Elisa Kit

Sharraxaadda Wax soo saarka:

Cudurka 'Newcastle Cuntody Kit waa aalad cudur-aqoonno loogu talagalay ogaanshaha tayada ee tayada ee loo yaqaan' ailpodies 'ee unugyada cudurka cudurrada ee Newcastle (NDV) ee katirsan cudurka' serum '(NDV) ama muunadaha plasma ee digaagga. Farsamaynta Enzyme - Farsamaynta Tilmaanta Immelles ee Tilmaanta Assay (Elisa), xirmadaan ayaa bixiya hab xasaasi ah oo gaar ah oo loogu talagalay in lagu garto shimbiraha loo soo saaray cudurka loo yaqaan 'pattergen'. Qalabka sida caadiga ah waxaa ku jira dhammaan reages iyo qaybaha loo baahan yahay, sida taarikada loo yaqaan 'pre - taarikada dahaarka leh oo leh antigens gaar ah, kontaroolo, iyo u oggolaanshaha baaritaanka hufan ee hufnaanta loo yaqaan' sederaar '.

Mabda'a baaritaanka:

Qalabkan adeegsiga Habka ELISA, NDV Antigen waa Pre - oo ku dahaadhka ku yaal microplate. Marka baaritaanka la tijaabiyo, ku dar muunadda serum, ka dib gubashada, haddii ay jiraan NDV gaar ah, waxay ku dari doontaa antiunten-ka, iska tuur antibody-ka ka hortagga ah iyo waxyaabaha kale ee lagu maydho; Ka dib ku dar enzyme availate anti - NDV Monoclonal-ka antibody, ka hortag ah muunad ah oo muunad ah oo ka mid ah isku-darka antibody-ka monoclonal iyo pre / jarista kareemka ah; Tuur in aan la shaacin enzyme enzyme conjugate oo dhaqitaanka. Ku dar Substrate-ka TMB ee Micro - Wells, seenyaalaha buluuga ah ee ay sameeyeen enzyme catalysis-ka ayaa ku jirta qayb ka mid ah qayb ka mid ah antibody-ka ee muunad. 

Isticmaal:

Ogaanshaha antibody ee gaarka ah ee Newcastle Despalespilies

Kaydinta:Dhammaan reages-ka oo dhami waa in lagu kaydiyaa 2 ~ 8 ℃. Ha qaboojin.

Heerarka Fulinta:Heerka caalamiga ah.

Tusmada: