Mycoplasma Gallisepticm Ab Teko Kit (Elisa)

Ts'ebetso ea Elisa:

1) Nka pele - microplatete ea 'mala o mongata oa ho sebelisa chelete e' ngoe e le 'mala oa sampole), Liliba tse peli tsa taolo e ntle ka thoko. Eketsa 100μl Taolo e fosahetseng / e ntle ho liliba tsa eona. Shake bonolo, u se ke oa phatlalla, se koahele ebe u kenella ho 37 ℃ bakeng sa 30 min.

2) Tšela mokelikeli o tsoang lilibeng, eketsa 250 μl e kopantsoeng le e 'ngoe le e' ngoe e ntlafalitsoeng, tšela. Pheta 4 - makhetlo a 6, qetellong ea ho omella pampiring e monyang.

3) Kenya enzyme ea 100μl e kopantsoeng le e 'ngoe e phehloletsoeng' moho, ts'oara butle, koahela Andincubute ho 37 ℃ bakeng sa 30 min.

4) Pheta mohato oa 2 (ho hlatsoa). Hopola Pat ho omisa pampiring e monya qetellong.

5) Kenya 100μl sublicate ho e 'ngoe le e' ngoe, kopanya hantle, u itshokele ka makhetlo a 10 Antarrk ka37 ℃ lefifing.

6) Eketsa 50μl ea tharollo ea ho emisa ka lebaka le ka 'ngoe,' me u lekanye ka lebaka le ka har'a 10 mets.

Tlhaloso ea Sehlahisoa:

Mycoplasma Gallinod antibod antibod entibod elisa kit e thehiloe ho sa tokollo e sa tobang e sa tloaelehang Ha serume sa sampole e na le li-antibodies tse khethehileng, li tla tlama ho li-antigen ka lipolaneteng. Hlatsoa li-antibodies tse sa thabiseng le likarolo tse ling. Ebe u eketsa enzyme e itseng. Kamora ho incubation le ho hlatsoa, ​​eketsa TBB e tlatselletsa. Boitšoaro ba mebala bo tla hlaha, bo lekantsoe ke mofuta oa sebopeho (450 nm).

Kopo:

Kit ena e sebelisoa ho bona Mycoplasma Gallinopticum (MG) Antibort arum ea likhoho

Boloka: Ho boloka ho 2 - 8 ℃ lefifing.

Melao e Mabetsang:Tekanyetso ea Machabeng.