I-Haemophilis Parasuis Viki (RT - PRR)
Inkcazo yemveliso:
Indredies |
Numbhali |
Ishumi - Ibhokisi yeHole yokupakisha |
2 × Qpcr MAGMIX |
I-90408 |
I-500s (i-brown) |
Duent ye-frioresceccec ye-PCR Iitemplate |
I-180701 |
I-1 ml (i-tyheli) |
Umxube wePrim Primer ye-bovine Humbersvirus |
14 - I-51800yyw |
I-100μl (intloko emhlophe) |
Ulawulo oluqinisekileyo lweBovine Humbesvirus PCR (1 × 10e8 / μl) |
14 - I-51800pc |
I-50μ (i-red cap) |
Isisombululo se-vissis se-LNA ye-DNA Virus (ipakethi yetyala) |
3674A |
Izihlandlo ezili-15 (9ml) |
Ingcaciso yeMveliso:
Le khit ilungele ukubuyisela umva ukuqalisa kweesampulu ezahlukeneyo zeRNNA kunye ne-prc sught. Isebenzisa i-M - mlv ye-reverse yokukhupha, ivumela isizukulwana semveliso ende ende. Ukongeza, kumxube we-20 μl yokubuyisa i-Precripripr yoRhwebo kunye ne-50 μl ye-PCR PRCE, inika inani elaneleyo lemveliso ye-PCR ye-DOWNSCAM YOKUGQIBELA. I-enzymes equkayo kule khit ingeniswa, i-enzyme ye-RORTE ingeniswa i-M - MLV, ikhokelela kwiNkqubo yeCena ephezulu kunye nokugcina ulwazi olupheleleyo lwe-CDNA kunye nokugcina ulwazi olupheleleyo lwe-CDNA kunye nokuGqibezela ngokupheleleyo ulwazi? I-Treator ekhethekileyo esetyenziswa ngexesha lokutshintsha kwenkqubo yokuguqula incitshiswa ngokufanelekileyo ingozi yokungaphumeleli ngenxa yokungaphumeleli ngenxa yokungcola kwe-cnase. Le khiti ilula kwaye iyakhawuleza ukuyisebenzisa, iyenza isebenze ngokubanzi kwi-CDNA i-CDNA i-CDNA i-CDNA I-CDNAYS EYENZIWEYO KWI-MALECRUR YOKUGQIBELA IIMPAWU ZOPHULO.
Isicelo:
Le khiti ilula kwaye iyakhawuleza ukuyisebenzisa, iyenza isebenze ngokubanzi kwi-CDNA i-CDNA i-CDNA i-CDNA I-CDNAYS EYENZIWEYO KWI-MALECRUR YOKUGQIBELA IIMPAWU ZOPHULO.
Ukugcinwa: Gcina nge-20 ° C. Kuphephe ukukhululeka rhoqo - Imijikelezo yethayile.
Imigangatho yolawulo:Umgangatho wehlabathi.