Intsholongwane kaGawulayo (i-GP41, GP36) │ IVenkile yeGcienedefinintle yoLuntu (i-GP41 ne-GP36) i-chimeric antign

Ingcaciso yeMveliso:

Intsholongwane kaGawulayo, okanye intsholongwane kaGawulayo, yi-retrovirus ejolise kwiiseli zamajoni omzimba, ngakumbi i-CD4 - i-CD4 - i-TWS, ikhokelela kwintshabalalo okanye i-CEPROS, ikhokelela kwintshabalalo okanye intshabalalo. Oku kuqhubela phambili kwamajoni omzimba kubangela ukumiswa kwenkqubo yokuzikhusela, ukwenza ukuba abantu bathathe inxaxheba kusulelo olungenelelayo kunye nomhlaza othile. Intsholongwane idluliselwa ngokudibana negazi elosulelekileyo, i-semen, ulwelo lobufazi, kunye nobisi lwebele, kunye nonxibelelwano lwesini, kunye nomama, nakumama entsalela ekubeleni.

Izicelo ezicetyiswayo:

I-Lateraly I-Imunosassay, Elisa

Icetyiswa intlawulo:

Ukuze isicelo siphindwe kabini - Isandwich ye-Antigen yokufumana, i-AIRE00511 kunye ne-AI00506 yokufakwa.

Inkqubo ye-Buffer:

I-50mm Tris - I-HCL, 0.15m nacl, pH 8.0

Hlalani:

Nceda ubone isatifiketi sohlalutyo (i-COA) ethunyelwa kunye neemveliso.

Ukuhambisa ngenqanawe:

Iiproteni zeProtesi kwifom engamanzi zithuthwa kwifom ye-frozen ene-ice eluhlaza.

Ukugcinwa:

Kwindawo yokuhlala yexesha elide, imveliso iyazinza iminyaka emibini ngokugcinwa kwi - 20 ℃ okanye isezantsi.

Nceda usebenzise imveliso (ifomu engamanzi) kwiiveki ezi-2 ukuba igcinwe kwi-2 - 8 ℃.

Nceda uphephe ukukhululeka okuphindaphindiweyo - Imijikelezo yethayile.

Nceda unxibelelane nathi ngayo nayiphi na inkxalabo.

Imvelaphi:

Intsholongwane yamandla abantu, kukho i-genotypes ezine, m, n, n, n, n, n, nothungelwano oluphambili yi-NAOOMETERNEAMETER kwaye iphantse ibekwe. I-prombrane yangaphandle yentsholongwane yimvulophu ye-lipid eneproteni yentsholongwane GP120 kunye ne-GP41. I-GP41 yiproteyini ye-grtranene. I-GP120 ibekwe kumphezulu kwaye ibophelele kwi-GP41 ngokungathi kunjalo - Unxibelelwano olunamanzi. Ngaphakathi kukho iMatrix ye-STREP eyenziwe yiProtein P17 kunye ne-semi yakho - I-COPID ye-semical yenziwe yiProtein p24.