Methylated Septin 9 i-PCR yokufumanisa
Imveliso Inkcazelo:
I-Septin9 kunye ne-NDRGE4 ye-Geethy Reting Kit yinyani - Ixesha le-prc scey yokufunyanwa kwe-DNAMIRT ye-DNArmal DethyId kwi-DNA yeseli ekhutshelwe kwiplasma yomntu. Le kit isekwe kwi-TaqMantm DNA Pe Polymerase Ampharame kunye neFluorochrome - I-Libed Ingxelo ekhethekileyo yePREBE. Ukulandela ukuguqulwa kwe-suulfite ye-nuccootides ye-cytosine ye-cytosine ukuya kwi-UCCEIL, le khiti ifumana i-metyen ye-Septin9 kunye ne-NDRG4 kunye nolawulo lwangaphakathi, i-ACHB ukubeka iliso kwingqokelela yesampulu, i-ACHB ukubeka iliso kwingqokelela yesampulu, ukukhutshelwa kwe-DNA, kunye nokukhutshwa kwe-DNA, kunye ne-amunition.
Ezinye izinto eziyimfuneko:
1.Ukunyanzela i-PCR I-PRCR I-PRREY YOKUFUNDA I-Fami Qaphela: Ijelo le-Vic lingasetyenziselwa endaweni yeJoe kwizixhobo apho uJoe akakhange atshintshe.
Umxube we-2.vortex
3.Microsoft)
4.Pipettes
Iingcebiso ze-nultise nucle - Iingcebiso zasimahla zepayitha (iingcebiso zomhoqo (i-farrict
6.CoT PleCR PLACR
Isicelo:
Le khiti isetyenziselwa ukufunyanwa kwe-vitrotion ye-methylated Septin9 (i-MSEPT9) kunye ne-NDRG9) kunye ne-NDRG4 ye-NDRG4 kwi-DNA ikhutshwe kwiplasma yomntu.
Ukugcinwa: Ikhithi inokugcinwa kwi - 20 ° C kangangesithuba seenyanga ezili-12 ngaphambi kokuvulwa. Emva kokuvula, iiRegents zisebenza ubuncinci kwiinyanga ezi-6 ukuba zigcinwe - I-20 ° C.
Imigangatho yolawulo:Umgangatho wehlabathi.