Mycoplasma gallisepticum Ab Test Kit (ELISA)

I-MyCilplasma Gallieptimum i-AB Kit (Elisa)

Inkcazo emfutshane:

Igama eliqhelekileyo: I-Mycoplasma Galliepticum (MG) Anti yovavanyo lwe-Elisar

IKHODI: Uvavanyo lwezempilo yezilwanyana - Avian

Isampulu yovavanyo: I-Serum

Ukulungiselela isampulu

Igama lophawu: I-Colorcom

Ubomi beShelpu: iinyanga ezili-12

Indawo yemvelaphi: China

Inkcazo yemveliso: I-96 Wells / Kit


    Iinkcukacha zemveliso

    Iimpawu zeMveliso

    Inkqubo ye-ESASA:

    I-1) Thatha kwangaphambili - I-MICPE edityanisiweyo (ingangabikho ixesha lokusetyenziswa kwexesha njengenani lesampulu), yongeza i-100 μi-1 kakuhle kulawulo olubi, ii-2 Wells zolawulo olubi ngokwahlukeneyo. Yongeza i-100μl engalunganga / eyakhayo kwiqula layo. Shake kancinci, ungaphuzi, gubungela kwaye ubumbele nge-37 ℃ kwimizuzu engama-30.

    2) galela ulwelo ngaphandle kwequla, yongeza i-250 μl i-buffere ebilisiweyo ye-bility nganye, thula. Phinda u-4 - Amaxesha ama-6, kwi-PAT yokugqibela ye-PAT ukuze inyuke kwiphepha lokufumana.

    3) Yongeza i-100 i-enzyme ivumelana kwi-quot nganye, inyikima ngokuthambileyo, ikhava i-37 ℃ kwimizuzu engama-30.

    4) Phinda inyathelo 2 (ukuhlamba). Khumbula i-PAT ukuba yome kwiphepha le-alkerment.

    5) Yongeza i-100 ye-8μints nganye, xuba ngokufanelekileyo, jongana nemizuzu eli-10 i-Atdark At37 ℃ ebumnyameni.

    6) Yongeza i-50μl yesisombululo sokumisa kwi-HIV, kwaye ulinganise iziphumo ngaphakathi kwe-10 min.

     

    Ingcaciso yeMveliso:

    I-Mycoplasma Galliepticum (MG) Anti kit ye-E-Elisa isungulwa kwi-itassossay engathanga ngqo (i-Ifarect Elisa) .I-Antign idityaniswe kwiipleyiti. Xa i-serium yesampulu iqulethe ii-antinedies ezithile kwintsholongwane, ziya kubopha eAntign kwiipleyiti. Hlamba ii-antibodies ezingavakaliyo kunye nezinye izinto. Yongeza i-enzyme ethile congerme. Emva kokufakwa kunye nokuhlamba, yongeza i-substrate. Impendulo yemibala iyavela, ilinganiswe yi-spectraphotometer (450 NM).

     

    Isicelo:

    Le khit isetyenziselwa ukufumana i-MyCoplasma Gallieptum (MG) i-antidy serible i-inkqo-roum ye-MyCoplasma ye-Mycoppepticum kwaye incedise ekufumaneni inkukhu yenkukhu.

    Ukugcinwa: Ukugcina nge-2 - 8 ℃ ebumnyameni.

    Imigangatho yolawulo:Umgangatho wehlabathi.

  • Ngaphambili:
  • Okulandelayo:

    • iimveliso ezinxulumene noko