Haemophilus parasuis Test Kit (RT-PCR)

I-Haemophilus Parasuis Test Kit (RT - PCR)

Incazelo emfushane:

Igama elijwayelekile: I-Haemophilus Parasuis Test Kit (RT - PCR)

Isigaba: Ukuhlolwa Kwezempilo Yezilwane - Imfuyo

Okuhlosiwe kokutholwa: I-haemophilus parasuis antibody

Isikhathi sokufunda: 5 ~ 10 imizuzu

Igama lomkhiqizo: colorcom

Impilo yeshelufu: izinyanga eziyi-12

Indawo yemvelaphi: China


    Imininingwane Yomkhiqizo

    Amathegi Omkhiqizo

    Ukucaciswa komkhiqizo:

    Inggedient

    NUmber

    I-Ten - Hole Box Paking

    2 × QPCR magicmix

    90408

    I-500μL (cap brown)

    I-DILUED ye-Fluorescent PCR templates

    I-180701

    I-1 ml (ephuzi cap)

    Ingxube ye-Primer ye-Primer ye-Bovine Herpesvirus

    14 - 51800YW

    I-100μl (i-cap emhlophe)

    Ukulawulwa okuhle kwe-Bovine Herpesvirus PCR (1 × 10e8 Amakhophi / μL)

    14 - 51800PC

    I-50μL (cap red)

    Isixazululo se-Viral lysis se-DNA Virus (Iphakethe Lecala)

    3674a

    Izikhathi eziyi-15 (9ml)

     

    Ukuchazwa Komkhiqizo:

    Le khithi ilungele ukuphendulwa kokubhaliwe okubuyiselwe emuva kwamasampula ahlukahlukene we-RNA kanye ne-PCR amplification elandelayo. Isebenzisa i-M - MLV yokuphendula umbhalo wokuphambuka, okwenza isizukulwane semikhiqizo emide ye-CDNA. Ngokwengeziwe, ukuhlanganiswa okubhaliwe kwe-20 μL Reverse real reaction kanye nevolumu yokuphendula engu-50 Ama-enzyme afakiwe kule kit angeniswa ngaphakathi, nge-RT enzyme eba yi-M - MLV engenisiwe, okuholela ekutholeni imicu ende ye-CDNA nokugcinwa okuphelele kolwazi lwezakhi! I-rnase invibitor ethize esetshenziswe ngesikhathi senqubo yokubhalwa kabusha yehlisa ngempumelelo ingozi yokwehluleka kokuhlola ngenxa yokungcola kwe-rnase exegrenous. Le khithi ilula futhi ishesha ukuyisebenzisa, okwenza kusetshenziswe kabanzi nge-CDNA Cloning kanye nokutholwa kohlobo lwe-gene ekuhlolweni kwe-molecular biology.

     

    Isicelo:

    Le khithi ilula futhi ishesha ukuyisebenzisa, okwenza kusetshenziswe kabanzi nge-CDNA Cloning kanye nokutholwa kohlobo lwe-gene ekuhlolweni kwe-molecular biology.

    Isitoreji: Gcina ngo-20 ° C. Gwema uqhwa njalo - imijikelezo emincane.

    Izindinganiso Ezokuphatha:Indinganiso yamazwe omhlaba.



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