Methylated Septin 9 PCR Detection Kit

I-methylated septin 9 PCRET KIT

Incazelo emfushane:

Igama elijwayelekile: I-methylated septin 9 PCRET KIT

Isigaba: Iphoyinti lokuhlolwa kokunakekelwa (POCT) - Ukuhlolwa kwe-molecular Diagnostic

Isampula yokuhlola: I-DNA

Ukucaciswa: Akukho mvume yokuqashwa kwesiphambano lapho kuhlolwa ikhithi.

Igama lomkhiqizo: colorcom

Impilo yeshelufu: izinyanga eziyi-12

Indawo yemvelaphi: China

Ukucaciswa komkhiqizo: 96t


    Imininingwane Yomkhiqizo

    Amathegi Omkhiqizo

    Umphumela Incazelo:

    I-sepnin9 ne-gerg4 gene methylation detection dection deal iyona engokoqobo - isikhathi PCR Assay sokutholwa kwe-DNA methylation engajwayelekile ye-DNA methylation kwi-Celt Free DNA ekhishwe ku-Plasma yabantu. Le kit isuselwa ku-taqmantm DNA polymerase amplification kanye ne-fluoroocochrome - ukubikwa okushiwo okucacisiwe. Ukulandela ukuguqulwa kwe-bisulfite kwama-nucleotides we-cytosine angafakwanga e-Uracil, le khithi ithola i-methylation ye-septin9 ne-ndrg4 yofuzo kanye nokulawulwa kwangaphakathi, ukukhishwa kwe-DNA, kanye nokukhulisa.

     
    Ezinye izinto ezidingekayo:

    Ithuluzi le-1.FLurescence PCR likwazi ukufundwa nge-FAM (494nm eliphezulu ukumuncwa kwesibalo, ukuphuma okuphezulu kwe-518NM) kanye noJoe (520nm ephezulu ukumunca, ukuphuma okuphezulu kwe-545nm, ukuphuma okuphezulu kwe-545nm). QAPHELA: Isiteshi se-VIC singasetshenziswa esikhundleni sikaJoe ku-Amathuluzi uJoe angakaze alinganiswe.

    I-2.Vortex Mixer

    3.Microcentrifuge

    Ama-4pipette

    I-5.State Nuclease - Amathiphu we-Pipette wamahhala (Amathiphu wokuvimba anconyiwe) kanye namashubhu we-microthuge

    6) ahambisane nepuleti ye-PCR

     Isicelo:

    Le kit isetshenziselwa ukufaneleka ekutholweni kwe-vitro ye-methylated septin9 (MSEPRT9) kanye nohlobo lwe-ndrg4 e-cell free DNA ekhishwe ku-plasma yabantu.

    Isitoreji: Ikhithi ingagcinwa ku- - 20 ° C isikhathi sezinyanga eziyi-12 ngaphambi kokuvula. Ngemuva kokuvula, ama-reagents avumelekile okungenani ezinyangeni ezi-6 uma zigcinwe ku- - 20 ° C.

    Izindinganiso Ezokuphatha:Indinganiso yamazwe omhlaba.

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