Mycoplasma gallisepticum Ab Test Kit (ELISA)

I-Mycoplasma Gallopppicum ab Test Kit (ELISA)

Incazelo emfushane:

Igama elijwayelekile: I-Mycoplasma Galleppicum (MG) Antibody Elisa Test Kit

Isigaba: Ukuhlolwa Kwezempilo Yezilwane - Avian

Isampula lokuhlola: i-serum

Ukulungiswa kwesampula: Thatha isilwane lonke, wenze i-serum ngokuya ngezindlela ezijwayelekile, i-serum kufanele icace, ayinakho hemolysis.

Igama lomkhiqizo: colorcom

Impilo yeshelufu: izinyanga eziyi-12

Indawo yemvelaphi: China

Ukucaciswa komkhiqizo: Imithombo engama-96 / kit


    Imininingwane Yomkhiqizo

    Amathegi Omkhiqizo

    Inqubo ye-ELISA:

    1) Thatha i-Pre - I-COECATICLATE (ingakwazi ukuqiniseka ngokusetshenziswa kwesikhathi ngakunye ubungako besampula), engeza i-serum eyi-100μL ehlanganisiwe kwimithombo yamasampula, okwamanje i-1 ngokulawulwa okuhle ngokwengeziwe. Engeza ukulawulwa okungalungile kwe-100μl emithonjeni yawo. Shake kancane, ungachithi, ukumboza futhi ufake ku-37 ℃ imizuzu engama-30.

    2) uthele uketshezi ngaphandle kwemithombo, engeza i-buffer engu-250 μl ehlanganisiwe emthonjeni ngamunye, uthele. Phinda u-4 - amahlandla ama-6, ekugcineni kwe-pat ukuze ome ephepheni lokumunca.

    3) Faka i-100μL enzyme conjugate komunye umthombo ngamunye, unikine kancane, ukumboza i-ancucubate ku-37 ℃ imizuzu engama-30.

    4) Phinda Isinyathelo 2 (ukugeza). Khumbula uPat ukuze ome ephepheni lokumunca ekugcineni.

    5) Faka i-100μl substrate kulowo nalowo nalowo, hlanganisa kahle, phenduke imizuzu eyi-10 i-AtDark AT37 ℃ ebumnyameni.

    6) Faka i-50μL yesisombululo sokumisa emthonjeni ngamunye, bese ukala umphumela ngaphakathi kwamaminithi ayi-10.

     

    Ukuchazwa Komkhiqizo:

    I-MyCoplasma Gallepticum (MG) Antibody Elisa Kit isuselwe e-Enzymaticmmmoassass engaqondile (elisa elingaqondile) .I-Antigen ehlanganiswe ngamapuleti. Lapho i-serum yesampula iqukethe ama-antibodies athile ngokumelene negciwane, azobopha ama-antigen kumapuleti. Geza ama-antibodies angavikeleki nezinye izinto. Ngemuva kwalokho engeza umhlanganisi othile we-enzyme. Ngemuva kokufakwa nokugeza, engeza i-tmb substrate. Ukuphendula okugqamile kuzovela, kulinganiswe nge-spectrophotometer (450 nm).

     

    Isicelo:

    Le kit isetshenziselwa ukuthola i-MycoPlasma Gallepticum (MG) Antibody in Chicken Serum, ukuhlola isimo se-antiboplasma nge-Mycoplasma Gallosppicum (MG) umuthi wokugomela inkunkuma yenkukhu nokusiza ukuthola inkukhu ye-serogical.

    Isitoreji: Ukugcina ngo-2 - 8 ℃ ebumnyameni.

    Izindinganiso Ezokuphatha:Indinganiso yamazwe omhlaba.

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