Isifo seNewcastle Isifo Antibody Elisa Kit

Ukuchazwa Komkhiqizo:

Isifo seNewcastle isifo antibody Elisa Ithuluzi liyithuluzi lokuxilonga elenzelwe ukutholwa kwamasosha omzimba akhethekile kwi-antibodies virus (NDV) kumasampula e-serum noma ama-plasma avela ezinkukhu. Isebenzisa i-enzyme - I-Immunosorbent Assay (ELISA) inqubo, le kit inikeza indlela ebucayi neqondile yokuhlonza izinyoni ezivezwe kwi-pathogen. I-kit ngokuvamile ifaka zonke izimpendulo ezidingekayo kanye nezinto, ezifana neza kuqala - amapuleti ahlanganisiwe anama-antigen athile, izilawuli, kanye ne-enzyme ebonakalayo, evumela ukuhlolwa okusebenzayo nokunembile kwizilungiselelo zelebhu.

Isimiso sokuhlolwa:

Le khithi isebenzisa i-block elisa indlela, i-NDV Antigen yi-Pre - ihlanganiswe ku-microplate. Lapho uvivinya, engeza isampula ye-serum ebhululiwe, ngemuva kokufakwa, uma kune-antibody ethize ye-NDV, izohlanganisa ne-antigen yangaphambili - lahlalisa i-antiigen nezinye izingxenye ngokugeza; Ngemuva kwalokho engeza i-enzyme ivele ilwele i-anti - I-NDV Monoclonal antibody, i-antibody in Tamp block inhlanganisela ye-monoclonal antibody ne-pre - i-antigen; Lahla umjovo we-enzyme ongaguquki ngokugeza. Ngeza i-TMB Substrate emicro - Wells, isiginali eluhlaza okwesibhakabhaka nge-enzyme catalysis isengxenyeni engqubuzana ye-antibody okuqukethwe ngesampula. 

Isicelo:

Ukutholwa kwe-antibody ethile yama-antibodies ama-newcastle deasespecties

Isitoreji:Onke ama-reagents kufanele agcinwe ngo-2 ~ 8 ℃. Ungabazi.

Izindinganiso Ezokuphatha:Indinganiso yamazwe omhlaba.

Okuqukethwe: