I-Porcine Pseudorabies Virus GD (PRV - GD) Antibody Elisa Kit

Ukuchazwa Komkhiqizo:

Le kit iqukethe ipuleti le-microtaiter elihlanganisiwe nge-PRV - I-GD, i-enzyme conjugates, kanye nokunye okuhambisana ne-reagents. Isebenzisa umgomo we-enzyme - I-Immunosorbent Assay (ELISA) ukuze ibone ama-antibodies abhekene ne-PRV - GD ku-porcine serum noma i-plasma. Ngesikhathi sokuhlolwa, lawula i-serum nesampula yokuhlola yengezwe epuletini. Ngemuva kokufakwa, uma isampula iqukethe i-PRV - ama-antibodies we-GD, azobopha ama-antigen ahlanganiswe epuletini le-microtiter. Ngemuva kokugeza izinyathelo zokususa izingxenye ezingavikelekile, kufakwa ama-enzyme conjugates, ahlanganisa ngqo i-antigen - izakhiwo ze-antibody epuleti. Ngemuva kokugeza futhi ukuze ususe ama-enzyme anvimbo, ama-reagents aphansi angezwa emithonjeni bese ephendula nge-enzyme - amalebula abhalwe, okuholela kombala oluhlaza okwesibhakabhaka. Ukuqina kombala kulingana ngqo nenani le-antibody elithile kwisampula. Ukusabela kunqanyulwa ngokungeza ikhambi lokuma, kuguqula ikhambi eliphuzi. Ukudonswa komthombo ngamunye kulinganiswa endaweni yakwa-450nm kusetshenziswa umfundi weplanethi ye-microtic (Microplate Reader) ukunquma ukuba khona kwe-PRV - ama-antibodies we-GD kusampula.

Isicelo:

Le as assay yakhelwe ukuthola ama-antibodies aphikisana nama-pseudorabies virus glycoprotein b (PRV - GD) ku-porcine serum noma ku-plasma. Ingasetjenziselwa ukuhlolwa kokusebenza kwe-Immeminological Virus ValagcIne ezingulubeni.

Isitoreji: 2 - 8 ° C

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