I-Porcine Toxoplasma Gondi Test Kit (ELISA)

Ukwakheka kwekhithi:

I-Microelisa Stripplate, HRP - I-realent realent, isampula yesampula, isixazululo sokugeza esigxilile esingu-20 × chromogen a, i-chromogen solution b, isixazululo sokuma, ukulawulwa okuhle.

Isimiso Sokuhlola:

Ikhithi yokuzimisela okufanelekile kwe-tox ab kusampula, yamukele i-tox sag1 ehlanjululwe kabusha i-antigen yokuhlanza i-antigen ehlotshisiwe ukuze ihlanganise ipuleti le-microtatiter, bese i-antigen ye-pipette ibe yimithombo, i-anti - I-Porcine Tox ihlangana ne-Horseradish Peroxidase (HRP). Geza futhi ususe okungekhona i-antibody nezinye izinto. Ama-antibodies acacisiwe e-antigen azobopha i-pre - camera antigen. Ngemuva kokugeza ngokuphelele, engeza isixazululo se-TMB substrate kanye nombala kukhula ngokuya ngenani le-tox ab. Ukusabela kunqanyulwa ngokungezwa kwesixazululo sokumisa kanye nokuqina kombala kulinganiswa endaweni yama-450 nm. Qhathanisa nenani le-cutoff ukwahlulela uma i-tox ab ikhona kwisampula noma cha.

Ukuchazwa Komkhiqizo:

Ikhithi yokuhlola ivumela ukunqunywa kwe-porcine toxoplasma antilody (tox -

Ithuluzi: I-Mircoplate Reader (iqukethe: 450nm, 630nm Wavelength), imishini ye-thermostatic (ama-37 degrees Celsius), i-pipette eguqukayo.

Isitoreji: Ikhithi izogcinwa [2 - 8 ℃]. I-striplate evulekile ye-microelisa ingagcinwa [2 - 8 ℃] futhi uvikele umnyombo. Sebenzisa okungenani izinyanga ezi-2.

Izindinganiso Ezokuphatha:Indinganiso yamazwe omhlaba.